SonaCell™ - Biofuel

Most of current R&D in renewable energy focuses on biological means, such as isolating new strains and/or modifying potential strains genetically, to enhance biofuel production. IntelligentNano’s novel SonaCell™ platform (patent-pending) utilizes sonodynamic technology to increase cellulase enzyme production by fungus like Trichoderma reesei for enhanced cellulose degradation and to increase metabolic activity of yeast, Saccharomyces cerevisiae for higher ethanol production. SonaCell™ can also increase algae cell biomass to increase biodiesel production.

After our SonaCell™ treatment, cellulase production by T. reesei was observed to increase by 16±6%. The same SonaCell™ treatment conditions induced 31±10% increase in ethanol production by S. cerevisiae, which implies a cumulative improvement of up to 52±16% in lignocellulosic bio-ethanol production. These findings offer a new approach to reducing the cost of producing fermentation-based bioethanol from USD $4.15/ L down to less than USD $3/L.

Extensive research and development are currently underway to evaluate the application of SonaCell™ to scaled-up bio-fermentation processes. 

biofuel2

                                                                                                   The Shake-Flask Fermentation Setup for Algae with Ultrasound Stimulation.

biofuel sample

 


(A) SEM picture of T. reesei sample that received LIPUS treatment during fermentation shows shrinking mycelia with crack openings on the mycelial wall. (B) SEM picture T. reesei sample that received no LIPUS treatment during fermentation shows more intact mycelia with far fewer crack openings on the mycelial wall. (C & D) SEM picture of S. cerevisiae that received LIPUS treatment and that did not, respectively. Both showed budding scars on the cells which imply multiplication of cell and it seemed that the LIPUS treated cells have more budding scars than the control does.